Functional analysis of a SoxE gene in the oriental freshwater prawn, Macrobrachium nipponense by molecular cloning, expression pattern analysis, and in situ hybridization (de Haan, 1849)
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cDNA Cloning and Expression Analysis of Gustavus Gene in the Oriental River Prawn Macrobrachium nipponense
The gustavus gene is required for localizing pole plasm and specifying germ cells. Research on gustavus gene expression will advance our understanding of the biological function of gustavus in animals. A cDNA encoding gustavus protein was identified and termed MnGus in the oriental river prawn Macrobrachium nipponense. Bioinformatic analyses showed that this gene encoded a protein of 262 amino ...
متن کاملMolecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense.
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. Real-time quantitative reverse polymerase chain rea...
متن کاملMolecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase.
Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is poorly understood. In t...
متن کاملEffect of thermal shocks on survival and safety responses of oriental river prawn, Macrobrachium nipponense (De Haan, 1849)
This study was performed to determine the severity of hormonal changes and the immune system of oriental river prawn (Macrobrachium nipponense) in the face of thermal stresses (heat and cold). For this purpose, 80 pieces of prawn with an average weight of 1.5 ±0.3g were prepared from Anzali wetland and in the laboratory after a rest period, under thermal stresses (from 15 ° C to 22 ° C) and col...
متن کاملMolecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense.
The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its expression in crustaceans. In this study,...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2019
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-019-1996-x